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Purified anti-Tubulin Beta 3 (TUBB3) Antibody

Pricing & Availability
Clone
AA10 (See other available formats)
Regulatory Status
RUO
Other Names
β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg
  • AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg
  • AA10_Purified_TUBB3_Antibody_2_WB_92313.jpg
    Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3), and NF-1 (lane 4) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal tubulin beta 3 (TUBB3) (clone AA10) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • AA10_Purified_TUBB3_Antibody_3_WB_060118
    Whole cell lysates (15 µg protein) from HEL (negative control), HeLa, NTERA-2, and A549 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-Tubulin Beta 3 (TUBB3) antibody, clone AA10. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TUBB3 mRNA expression levels (source: Human Protein Atlas).
  • AA10_Purified_TUBB3_Antibody_4_ICC_060118
    NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X‐100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti‐Tubulin Beta 3 (TUBB3) (AA10) antibody and followed by DyLight™ 594 goat anti‐mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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657401 25 µg 101 CHF
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657402 100 µg 229 CHF
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.25 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Herman JS, et al. 2018. Nat Methods. 379:15. PubMed
  2. Castroflorio E, et al. 2020. Cell Mol Life Sci. 78:3503. PubMed
  3. Baruah J, et al. 2020. Arterioscler Thromb Vasc Biol. 40:2244. PubMed
  4. Tonelli Gombalová Z, et al. 2020. Journal of Comparative Neurology. 528(15):2523-2550. PubMed
  5. Yamasaki AE, et al. 2020. Stem Cell Res. 49:102096. PubMed
  6. Jiang D, et al. 2019. Theranostics. 9:2395. PubMed
RRID
AB_2562569 (BioLegend Cat. No. 657401)
AB_2562569 (BioLegend Cat. No. 657402)

Antigen Details

Structure
450 amino acids with predicted molecular weight of 50 kD
Distribution

Cytosol

Function
Plays important roles in neuronal cell proliferation and differentiation
Interaction
Alpha tubulin, kinesin and dynein
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.

Gene ID
10381 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Tubulin beta-3
Specificity Alt (DOES NOT SHOW ON TDS):
Tubulin β-3 (TUBB3)
App Abbreviation (DOES NOT SHOW ON TDS):
WB,ICC
UniProt
View information about Tubulin beta-3 on UniProt.org

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Go To Top Version: 5    Revision Date: 08.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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