Ultra-LEAF™ Purified anti-mouse CD172a (SIRPα) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse brain membrane protein

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47⁴, in vivo blocking of dendritic cell migration³, enhancing of macrophage phagocytosis^2,4, immunohistochemical staining of cerebellum frozen sections¹, immunoprecipitation^2,4, and spatial biology (IBEX)^5,6.

Application References

(PubMed link indicates BioLegend citation)

1. Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
2. Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
3. Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
4. Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2832516 (BioLegend Cat. No. 144040)
AB_2832516 (BioLegend Cat. No. 144039)
AB_2832516 (BioLegend Cat. No. 144038)
AB_2832516 (BioLegend Cat. No. 144035)
AB_2832516 (BioLegend Cat. No. 144036)
AB_2832516 (BioLegend Cat. No. 144037)

Antigen Details

Structure

Type I transmembrane protein

Distribution

Monocytes, macrophages, myeloid cells, neuronal tissue

Function

Negative regulation of several biological processes

Interaction

PTPN6, PTPN11

Ligand/Receptor

CD47, SP-A, SP-D

Cell Type

Dendritic cells, Macrophages, Monocytes, Neutrophils

Biology Area

Cell Adhesion, Cell Biology, Immunology, Signal Transduction

Molecular Family

Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase

Antigen References

1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.

Gene ID

19261 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD172alpha

Specificity Alt (DOES NOT SHOW ON TDS):

CD172a (SIRPα)

App Abbreviation (DOES NOT SHOW ON TDS):

FC,IHC-F,IP,Block

UniProt

View information about CD172alpha on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis