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Pacific Blue™ anti-rat CD45 Antibody

Pricing & Availability
Clone
OX-1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte common antigen, LCA, T200, Ly-5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-1_PB_CD45_Antibody_102417
LOU rat splenocytes were stained with CD45 (clone OX-1) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
  • OX-1_PB_CD45_Antibody_102417
    LOU rat splenocytes were stained with CD45 (clone OX-1) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
202225 25 µg 94 CHF
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202226 100 µg 258 CHF
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Description

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6 fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Application References

(PubMed link indicates BioLegend citation)
  1. Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
  2. Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
  3. Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
  4. Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
  5. Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
  6. Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
  7. Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
  8. Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)
Product Citations
  1. He J, et al. 2023. Biomolecules. 13:. PubMed
  2. Thomson Z, et al. 2023. Nat Immunol. 1947:24. PubMed
  3. Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
  4. Areny-Balagueró A, et al. 2022. Pharmaceutics. 14:. PubMed
  5. Shimamura Y, et al. 2022. Commun Biol. 5:753. PubMed
RRID
AB_2721618 (BioLegend Cat. No. 202225)
AB_2721618 (BioLegend Cat. No. 202226)

Antigen Details

Structure
Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution

All hematopoietic cells except erythrocytes and platelets

Function
T cell receptor and B cell receptor signal transduction
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.

Gene ID
24699 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD45
Specificity Alt (DOES NOT SHOW ON TDS):
CD45
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 0    Revision Date: 10.26.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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