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- Clone
- A15126D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot of anti-α-Synuclein antibody (clone A15126D) and isotype-matched IgG2b control, binding to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant C-terminally truncated human α-synuclein (1-122). Lane 3: 20 µg of normal human brain lysate. Blots were incubated with 1 µg/ml of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-α-Synuclein, 117-122 antibody (clone A15126D) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 848601 | 25 µg | 89 CHF | ||||
| 848602 | 100 µg | 218 CHF | ||||
α-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 20 ng/ml - 3.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone does not cross-react with ß- or ?-synucleins using Western blotting or ELISA as detection method.
- RRID
-
AB_2650693 (BioLegend Cat. No. 848601)
AB_2650693 (BioLegend Cat. No. 848602)
Antigen Details
- Structure
- α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
- Distribution
-
Tissue sources: primarily nervous system, but lower expression in other tissues.
Distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular. - Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein
- Antigen References
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1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501:45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov. Disord. 26:2160. - Gene ID
- 6622 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- alpha-Synuclein 117-122
- Specificity Alt (DOES NOT SHOW ON TDS):
- α-Synuclein, 117-122
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-P,Direct ELISA
- UniProt
- View information about alpha-Synuclein 117-122 on UniProt.org
Related FAQs
Other Formats
View All α-Synuclein, 117-122 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-α-Synuclein, 117-122 | A15126D | WB,IHC-P,Direct ELISA |
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Purified anti-α-Synuclein, 117-122
Western blot of anti-α-Synuclein antibody (clone A15126D) an... 
IHC staining of purified anti-α-Synuclein, 117-122 antibody ...
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