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Purified anti-human CD227 (MUC-1) Antibody

Pricing & Availability
Clone
16A (See other available formats)
Regulatory Status
RUO
Other Names
Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
a-16A_Purified_CD227_Antibody_FC_1_100312
PHA-activated human peripheral blood lymphocytes (3 days) were stained with purified CD227 (clone 16A, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE and CD3 (clone HIT3a) Pacific Blue™.
  • a-16A_Purified_CD227_Antibody_FC_1_100312
    PHA-activated human peripheral blood lymphocytes (3 days) were stained with purified CD227 (clone 16A, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE and CD3 (clone HIT3a) Pacific Blue™.
  • b-16A_Purified_CD227_Antibody_FC_2_100312
  • c-16A_Purified_CD227_Antibody_ICC_080819
    BT474 breast cancer cell line was stained with anti-human CD227, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
  • d-16A_Purified_CD227_Antibody_IHC-P_08102022
    IHC staining using purified anti-MUC1 antibody (clone 16A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-EDTA, the tissue was incubated with 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The images above (left: secondary only control, right: Clone 16A, 10 µg/mL) were captured with a 40X objective. Scalebar = 50 µM.
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355601 25 µg 77 CHF
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355602 100 µg 141 CHF
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Description

Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Jurkat cells transfected with MUC1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1.

Application References

(PubMed link indicates BioLegend citation)
  1. Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed
Product Citations
  1. Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed
  2. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  3. Murad JP, et al. 2018. Front Immunol. 1.95. PubMed
  4. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  5. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
RRID
AB_2561642 (BioLegend Cat. No. 355601)
AB_2561642 (BioLegend Cat. No. 355602)

Antigen Details

Structure
500-1000 kD proteoglycan (glycosylated form)
Distribution

Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers

Interaction
Complexes with SOS1 and Grb2 in RAS signaling
Ligand/Receptor
ICAM-1 and Her2/neu (ErbB2)
Cell Type
Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339.
2. Agrawal B, et al. 1998. Cancer Res. 58:4079.
3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386.

Gene ID
4582 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD227
Specificity Alt (DOES NOT SHOW ON TDS):
CD227
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ICC,IHC-P
UniProt
View information about CD227 on UniProt.org
Go To Top Version: 5    Revision Date: 08.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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