Purified anti-human CD337 (NKp30) Antibody

Pricing & Availability
Clone
P30-15 (See other available formats)
Regulatory Status
RUO
Other Names
NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P30-15_Purified_072407
Human peripheral blood lymphocytes stained with purified P30-15, followed by anti-mouse IgG FITC
  • P30-15_Purified_072407
    Human peripheral blood lymphocytes stained with purified P30-15, followed by anti-mouse IgG FITC
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325202 100 µg 129 CHF
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Description

The p30-15 monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells. NKp30 enhances NK cell cytolysis of tumor cellts that are deficient in MHC class I molecules. NKp30 has been shown to associate with CD59 and TCRζ. The p30-15 antibody against human NKp30 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human NKp30
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References

(PubMed link indicates BioLegend citation)
  1. Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
  2. Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
  3. Markel G, et al. 2009. PLOS One 4:e5597. (Block)
  4. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  5. Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)
Product Citations
  1. Zhu Z, et al. 2022. Front Oncol. 12:814312. PubMed
  2. Gunesch JT, et al. 2020. Elife. 9: . PubMed
  3. Aarsund M, et al. 2022. Cancer Immunol Immunother. 71:2227. PubMed
  4. Wang H, et al. 2011. Int Immunopharmacol. 11:1732. PubMed
  5. Elssen C, et al. 2011. Blood. 118:2473. PubMed
  6. Joyce M, et al. 2011. Proc Natl Acad Sci U S A. 108:6223. PubMed
  7. Friedman D, et al. 2021. J Cell Sci. 134:. PubMed
  8. Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed
  9. Urlaub D, et al. 2017. J Immunol. 198:1944. PubMed
  10. Mace E, Orange J 2014. J Vis Exp. 85: 51100. PubMed
  11. Kim N, et al. 2020. Cancers (Basel). 12:. PubMed
  12. German Y, et al. 2021. Cell Reports. 36(1):109318. PubMed
  13. Crome SQ, et al. 2017. Nat Med. 1.213888889. PubMed
  14. Mao H, et al. 2010. J Virol. 84:4148. PubMed
RRID
AB_756106 (BioLegend Cat. No. 325202)

Antigen Details

Structure
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
Distribution

IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro

Function
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction
CD59, TCRζ
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Pende D, et al. 1999. J. Exp. Med. 190:1505.

Gene ID
259197 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD337
Specificity Alt (DOES NOT SHOW ON TDS):
CD337
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ELISA,Block
UniProt
View information about CD337 on UniProt.org
Go To Top Version: 2    Revision Date: 08.11.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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