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- Clone
- 7/4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GKPIPNPLLGLDST epitope tag
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Different amounts of V5 tagged proteins (Genescript, M0101) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal V5-tag (clone 7/4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. -
200ng V5 tagged protein was resuspended in PBS. Lane 1 was immunoprecipitated with 2 µg mouse IgG. Lane 2 was immunoprecipitated with 2 µg anti-V5-tag (clone 7/4) antibody. Lane 3 was immunoprecipitated with 2 µg anti-V5-tag (clone poly290238) antibody. Lane 4 was loaded with 50ng V5 tagged protein directly as a positive control. Western blot analysis was performed using mouse anti-V5 tag antibody (clone 7/4) and followed by HRP-anti-mouse IgG.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 680601 | 25 µg | 101 CHF | ||||
| 680602 | 100 µg | 265 CHF | ||||
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
IP - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2566386 (BioLegend Cat. No. 680601)
AB_2566386 (BioLegend Cat. No. 680602)
Antigen Details
- Structure
- 14 amino acid peptide (GKPIPNPLLGLDST)
- Function
- The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
- Biology Area
- Cell Biology
- Antigen References
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1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891. - Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- V5-tag
- Specificity Alt (DOES NOT SHOW ON TDS):
- V5-tag
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IP
- UniProt
- View information about V5-tag on UniProt.org
Other Formats
View All V5-tag Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-V5-tag | 7/4 | WB,IP |
| Direct-Blot™ HRP anti-V5-tag | 7/4 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-V5-tag
Different amounts of V5 tagged proteins (Genescript, M0101) ... 
200ng V5 tagged protein was resuspended in PBS. Lane 1 was i... -
Direct-Blot™ HRP anti-V5-tag
0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. ...
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