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AEC Chromogen Concentrate

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Regulatory Status
RUO
Other Names
Chromogen Detection Reagent
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925804 1 mL 74 CHF
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Description

The use of AEC in conjunction with peroxidase-based detection protocols results in the deposition of red chromogen deposits at reactions sites on tissue sections.

The 1 mL volume of 50x AEC chromogen provides enough material for 150 tests.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store this product between 2-8°C. AEC preparations should be stored in a manner which minimizes exposure to light.
Application

IHC
Recommended Usage

Each lot of this product is quality tested by immunohistochemistry.

The AEC chromogen concentrate is to be diluted with the AEC substrate buffer (Cat. No. 925903). The optimal working dilution should be determined for each specific assay condition.

Application Notes

AEC reaction product is soluble in alcohol. Do not use organic solvents after AEC staining.

Protocol:
1. Dilute AEC concentrate 1:50 with Substrate Buffer (Cat. No. 925903)
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount using aqueous mounting media
6. AEC induces a red precipitate with almost no background

Antigen Details

Gene ID
NA
Go To Top Version: 0    Revision Date: 03.04.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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