- Clone
- W17079A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- glyceraldehyde-3-phosphate dehydrogenase
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17079A_A488_GAPDH_Antibody_1_ICC_062618 W17079A_A488_GAPDH_Antibody_1_ICC_062618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17079A_A488_GAPDH_Antibody_1_ICC_062618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
HeLa cells were fixed with cold methanol for 10 minute and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 rat IgG2a, κ Isotype Ctrl antibody or with Alexa Fluor® 488 anti-GAPDH antibody (green) at (B) 1: 250 (2 µg/ml), (C) 1:500 (1 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilutions. Nuclei were counterstained with DAPI (blue). The images were captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
607905 | 25 µg | 136 CHF | ||||
607906 | 100 µg | 335 CHF |
GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein (161-335 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
This clone weakly reacts with rat GAPDH.
- RRID
-
AB_2734506 (BioLegend Cat. No. 607905)
AB_2734507 (BioLegend Cat. No. 607906)
Antigen Details
- Structure
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
- Distribution
-
Ubiquitously expressed, locate in cytoplasm and perinuclear region.
- Function
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
- Interaction
- Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
- Biology Area
- Cell Biology
- Antigen References
-
- Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
- Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
- Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
- Zheng L, et al. 2003. Cell. 114:255.
- Wang Q, et al. 2005. FASEB J. 19:869.
- Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
- Gene ID
- 5590 View all products for this Gene ID
- UniProt
- View information about GAPDH on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GAPDH Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GAPDH | W17079A | WB,ICC |
Direct-Blot™ HRP anti-GAPDH | W17079A | WB |
Alexa Fluor® 488 anti-GAPDH | W17079A | ICC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-GAPDH
Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Mol... HeLa cells were fixed with cold methanol for 15 minutes and... -
Direct-Blot™ HRP anti-GAPDH
Western Blot of human lysate using film imaging system. Whol... Western Blot of human lysate using digital imaging system. W... Western Blot of mouse lysate using digital imaging system. W... -
Alexa Fluor® 488 anti-GAPDH
HeLa cells were fixed with cold methanol for 10 minute and ...
Follow Us