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Alexa Fluor® 488 anti-HMB-45 Antibody

Pricing & Availability
Clone
HMB-45 (See other available formats)
Regulatory Status
RUO
Other Names
Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HMB-45_A488_Antibody_033122
Human paraffin-embedded melanoma tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-HMB-45 (clone HMB-45) (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
  • HMB-45_A488_Antibody_033122
    Human paraffin-embedded melanoma tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-HMB-45 (clone HMB-45) (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
  • HMB-45_A488_Antibody_080222
    Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-HMB-45 (clone HMB-45) (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
Compare all formats See Alexa Fluor® 488 spectral data
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911509 25 µg 171 CHF
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Description

The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Shen HQ, et al. 2013. Rom. J. Morphol. Embryol. 54(3):643û7 PubMed
RRID
AB_2924626 (BioLegend Cat. No. 911509)

Antigen Details

Structure
HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
Distribution

Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular.

Gene ID
6490 View all products for this Gene ID
UniProt
View information about HMB-45 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03.31.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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