Alexa Fluor 488 anti-Hsp70 Antibody anti-Hsp70

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Clone: W27 (See other available formats)
Regulatory Status: RUO
Other Names: Heat shock protein 70
Isotype: Mouse IgG2a, λ
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Product Citations: publications

W27_AF488_052710 — Human cervical cancer cell line, Hela, intracellularly stained with W27 Alexa Fluor® 488

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648003

25 tests

165 CHF

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648004

100 tests

347 CHF

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Description

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hsp70 protein from HeLa cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
RRID: AB_2119537 (BioLegend Cat. No. 648003)
AB_2119537 (BioLegend Cat. No. 648004)

Antigen Details

Structure: Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution: Ubiquitous, cytoplasmic and nuclear
Function: Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References: 1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571.
2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425.
3. Bukau B, et al. 1998. Cell 92:351.
Gene ID: 3308 View all products for this Gene ID
UniProt: View information about Hsp70 on UniProt.org

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Other Formats

View All Hsp70 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Hsp70	W27	WB,ICFC,ICC
Alexa Fluor® 488 anti-Hsp70	W27	ICFC
Direct-Blot™ HRP anti-Hsp70	W27	WB

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 12.15.2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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