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Alexa Fluor® 488 anti-human CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
9-4D2-1E4 (See other available formats)
Regulatory Status
RUO
Workshop
V MA199
Other Names
M-CSFR,CSF-1R, FMS, CSF-1 Receptor
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
9-4D2-1E4_A488_CD115_CSF-1R_Antibody_012116
Human peripheral blood monocytes were stained with CD115 (clone 9-4D2-1E4) Alexa Fluor® 488 (filled histogram) or rat IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • 9-4D2-1E4_A488_CD115_CSF-1R_Antibody_012116
    Human peripheral blood monocytes were stained with CD115 (clone 9-4D2-1E4) Alexa Fluor® 488 (filled histogram) or rat IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
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347311 25 tests 153 CHF
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347312 100 tests 340 CHF
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitinylation of intracellular residues. The natural ligands of CD115 include M-CSF and IL-34. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature.  Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.

Application References

(PubMed link indicates BioLegend citation)
  1. Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
RRID
AB_2566757 (BioLegend Cat. No. 347311)
AB_2566757 (BioLegend Cat. No. 347312)

Antigen Details

Structure
Single-pass type I membrane protein and is 150 kD.
Distribution

Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.

Ligand/Receptor
Macrophage Colony Stimulating Factor (M-CSF) and IL-34.
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sherr CJ, et al. 1989. Blood 73:1786
2. Roussel MF, et al. 1991. Nature 353:361.
3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924.

Gene ID
1436 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

Why do I have a weak CD115 staining?

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.

Go To Top Version: 1    Revision Date: 01.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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