- Clone
- 33.1 (Ab33) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tie2, TEK
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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334208 | 100 tests | 293 CHF |
CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant extracellular domain of human Tie2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA
-
Application References
(PubMed link indicates BioLegend citation) -
- Peters KG, et al. 1998. Br. J. Cancer. 77:51.
- Wong AL, et al. 1997. Circ. Res. 81:567.
- Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
- Product Citations
-
- RRID
-
AB_1186028 (BioLegend Cat. No. 334208)
Antigen Details
- Structure
- 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
- Distribution
-
Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
- Function
- Angiogenesis, hemotopoiesis, monocytes chemotaxis
- Ligand/Receptor
- Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors, Monocytes
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1.De Palma M, et al. 2005. Cancer Cell. 8:211
2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168
3. Hsu HC, et al. 2000. Blood. 96:3757
4. Arai F, et al. 2004. Cell. 118:149 - Gene ID
- 7010 View all products for this Gene ID
- UniProt
- View information about CD202b on UniProt.org
Related FAQs
Other Formats
View All CD202b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | FC,IP,WB,IHC-F,ELISA |
Biotin anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | FC |
PE anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | FC |
Alexa Fluor® 488 anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | FC |
Alexa Fluor® 647 anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | FC |
TotalSeq™-A0588 anti-human CD202b (Tie2/Tek) | 33.1 (Ab33) | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD202b (Tie2/Tek)
Human endothelial cells (HUVEC) stained with purified AB33, ... -
Biotin anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with biotinylate... -
PE anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 PE -
Alexa Fluor® 488 anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 Alexa ... -
Alexa Fluor® 647 anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 Alexa ... -
TotalSeq™-A0588 anti-human CD202b (Tie2/Tek)
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