- Clone
- IA6-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_IA6-2_AF488_1_022712 1_IA6-2_AF488_1_022712](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/IA6-2_AF488_1_022712.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and IgD (clone IA6-2) Alexa Fluor® 488 (top) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (bottom). -
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Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: IgD (blue) in Cycle 2, PD-1 (green) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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348215 | 25 tests | 118 CHF | ||||
348216 | 100 tests | 265 CHF |
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IgD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
SB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.
- Additional Product Notes
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Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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Application References
(PubMed link indicates BioLegend citation) -
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_11150397 (BioLegend Cat. No. 348215)
AB_11150595 (BioLegend Cat. No. 348216)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
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Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
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1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32. - Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
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It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
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Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
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Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
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Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
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In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
PerCP anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 605™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 700 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Purified anti-human IgD
Human periperhal blood lymphocytes stained with purified IA6... -
PE anti-human IgD
Human periperhal blood lymphocytes stained with IA6-2 PE and... -
Biotin anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 (c... -
FITC anti-human IgD
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PerCP/Cyanine5.5 anti-human IgD
Human peripheral blood lymphocytes stained with CD19 APC and... -
PE/Cyanine7 anti-human IgD
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Alexa Fluor® 488 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 Br... Confocal image of human lymph node sample acquired using the... -
APC/Cyanine7 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 Br... -
Brilliant Violet 510™ anti-human IgD
Human peripheral blood lymphocytes were stained with IgD (cl... -
APC anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Pacific Blue™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Brilliant Violet 421™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
Purified anti-human IgD (Maxpar® Ready)
Human PBMCs stained with 147Sm-anti-CD20 (2H7) and 146Nd-ant... -
PE/Dazzle™ 594 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
APC/Fire™ 750 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 785™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
TotalSeq™-A0384 anti-human IgD
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TotalSeq™-C0384 anti-human IgD
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TotalSeq™-B0384 anti-human IgD
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PE/Cyanine5 anti-human IgD
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0384 anti-human IgD
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Spark UV™ 387 anti-human IgD
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human IgD
Typical results from human peripheral blood lymphocytes stai...
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