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Alexa Fluor® 488 anti-human IL-17F Antibody

Pricing & Availability
Clone
Poly5166 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17F, IL-17F
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5166_1_020711
PMA + ionomycin-stimulated (6 hours in the presence of monensin) human peripheral blood lymphocytes surface stained with CD3 APC, fixed and permeabilized and stained with IL-17F (clone Poly5166) Alexa Fluor® 488 (top) or blocked by pre-incubation with purified IL-17F (Poly5166) (bottom).
  • Poly5166_1_020711
    PMA + ionomycin-stimulated (6 hours in the presence of monensin) human peripheral blood lymphocytes surface stained with CD3 APC, fixed and permeabilized and stained with IL-17F (clone Poly5166) Alexa Fluor® 488 (top) or blocked by pre-incubation with purified IL-17F (Poly5166) (bottom).
  • Poly5166_2_020711
See Alexa Fluor® 488 spectral data
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516603 25 tests 147 CHF
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516604 100 tests 347 CHF
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Description

IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions and in a variety of auto-immune diseases.

The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F. It is a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.

BioLegend's purified Poly5166 antibody is IL-17F specific and can be paired with biotinylated Poly5166 to recognize IL-17F or with biotinylated anti-human IL-17A clone BL23 to recognize the IL-17A/F heterodimer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The pure Poly5166 (Cat. No. 516601) antibody is useful as the capture antibody in a sandwich ELISA assay.  Poly5166 can be used as a poly-poly pair with biotinylated Poly5166 to recognize human IL-17F, or it can be used as a poly-mono pair with biotinylated BL23 (Cat. No. 512705) to recognize the human IL-17A/F heterodimer (Cat. No. 580609).

Product Citations
  1. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  2. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  3. Nguyen DX, et al. 2021. iScience. 24(9):102973. PubMed
  4. Hernndez DC, et al. 2021. Immunity. :. PubMed
  5. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  6. Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
RRID
AB_10720816 (BioLegend Cat. No. 516603)
AB_10720816 (BioLegend Cat. No. 516604)

Antigen Details

Structure
Homodimer
Distribution

T cells, Th17 cells, memory CD4 T cells, gamma delta T cells, and epithelial cells, activated human mast cells, and basophils

Function
IL-17F induces various cytokines, chemokines, and adhesion molecules, including IL-6, IL-8, CXCL1, CXCL5, transforming growth factor-b (TGF-b), CCL2, G-CSF, granulocyte macrophage-colony forming factor (GM-CSF) and intracellular adhesion molecule-1 (ICAM-1), in airway epithelial cells, vein endothelial cells, and fibroblasts.
Interaction
IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
Ligand/Receptor
IL-17F utilizes IL-17RA and IL-17RC
Cell Type
Basophils, Epithelial cells, Mast cells, T cells, Th17
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Starnes T, et al. 2001. J. Immunol. 167:4137.
  2. Kawaguchi M, et al. 2001. J. Immunol. 167:4430.
  3. Hizawa N, et al. 2006. Clin Experimental Allergy 36:1109.
  4. Seiderer J, et al. 2008. Inflamm Bowel Dis. 14:437.
  5. Wu X, et al. 2010 Int J Cancer 127:86.
Gene ID
112744 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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