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Alexa Fluor® 488 anti-mouse CD147 Antibody

Pricing & Availability
Clone
OX-114 (See other available formats)
Regulatory Status
RUO
Other Names
Basigin
Isotype
Rat IgG1, κ
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Product Citations
publications
OX-114_A488_CD147_Antibody_061318
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 2.5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 488 (green) and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • OX-114_A488_CD147_Antibody_061318
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 2.5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 488 (green) and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • OX-114_A488_CD147_Antibody_1_121018
    Dissected C57/B6 mouse testis was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 488 (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
  • OX-114_A488_CD147_Antibody_2_050819
    Formalin-fixed, 300 micron-thick mouse testis section was blocked, permeabilized and stained overnight with CD106 (clone 429, MCVAM.A) Alexa Fluor® 647 (red), and CD147 (clone OX-114) Alexa Fluor® 488 (green) both at 5 µg/mL, optically cleared, and analyzed at 225 µm imaging depth on a confocal microscope. Watch the video.
  • OX-114_A488_CD147_Antibody_4_091721.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse CD147 Antibody (clone OX-114) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD117 (c-Kit) Antibody (clone 2B8) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD106 Antibody (clone 429 (MVCAM.A)) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
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Cat # Size Price Quantity Check Availability Save
123717 25 µg 147 CHF
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123718 100 µg 358 CHF
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Description

CD147 is a widely expressed membrane glycoprotein and reported to be implicated in cell adhesion, embryonic development, T cell activation and many other functions. It is expressed in thymocytes and many other cells, including epithelial and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone OX-114 has been shown to bind both two and three domain forms of mouse CD1471.

Application References

(PubMed link indicates BioLegend citation)
  1. Hanna SM, et al. 2003. BMC Biochemistry 4:17.
Product Citations
  1. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
RRID
AB_2734189 (BioLegend Cat. No. 123717)
AB_2734189 (BioLegend Cat. No. 123718)

Antigen Details

Structure
Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
Distribution

CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.

Function
CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
Ligand/Receptor
cyclophilin
Cell Type
Macrophages, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hanna SM, et al. 2003. BMC Biochemistry 4:17
2. Yurchenko V, et al. 2005. Immunology 117:301
3. Renno T, et al. 2002. J. Immunol. 168:4946

Gene ID
12215 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org
Go To Top Version: 4    Revision Date: 04.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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