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Alexa Fluor® 488 anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
1_GL7_AF488_GL7_Antibody_FC_1_032514
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and GL7 (clone GL7) Alexa Fluor® 488 (top) or rat IgM, κ Alexa Fluor® 488 isotype control (bottom).
  • 1_GL7_AF488_GL7_Antibody_FC_1_032514
    BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and GL7 (clone GL7) Alexa Fluor® 488 (top) or rat IgM, κ Alexa Fluor® 488 isotype control (bottom).
  • 2_GL7_AF488_GL7_Antibody_FC_2_032514
  • GL7_A488_GL7_Antibody_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) Alexa Fluor® 488 (green), CD3 (clone 17A2) Alexa Fluor® 594 (red) and B220 (clone RA3-6B2) Alexa Fluor® 647 (blue) overnight at 4°C. The image was captured by 10X objective.
  • MariaPopescu.jpg
    Tetanus toxin immunized mouse spleen, frozen in OCT and sliced, then labeled with B220/CD45R Alexa Fluor® 700 (blue), anti-mouse CD169 Alexa Fluor® 647 (magenta, clone 3D6.112), anti-mouse CD4 Alexa Fluor® 594 (clone GK1.5) and anti-mouse/human GL7 Alexa Fluor® 488 (red, clone GL7). Image generously submitted to the 2017 Cell Life Imaging Competition by Maria Popescu from State University of New York Upstate Medical University.
Compare all formats See Alexa Fluor® 488 spectral data
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144611 25 µg 77 CHF
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144612 100 µg 194 CHF
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Jiang W, et al. 2021. Sci Rep. 11:1864. PubMed
  2. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  3. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  4. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  5. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  6. Lütge M, et al. 2023. Nat Immunol. . PubMed
  7. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  8. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  9. Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed
  10. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  11. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  12. Lee GJ, et al. 2020. Oncoimmunology. 9:1746553. PubMed
  13. Yang Z, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.04.035. PubMed
  14. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  15. Liu Y, et al. 2019. Nat Commun. 10:324. PubMed
  16. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
  17. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  18. Park JS, et al. 2022. J Transl Med. 20:85. PubMed
  19. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  20. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
RRID
AB_2563284 (BioLegend Cat. No. 144611)
AB_2563284 (BioLegend Cat. No. 144612)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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