Alexa Fluor 488 anti-Nanog Antibody anti-Nanog

Pricing & Availability

Clone: 23D2-3C6 (See other available formats)
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

23D2-3C6_A488_Nanog_Antibody_1_051419 — NCCIT pluripotent embryonal carcinoma cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml anti-Nanog (clone 23D2-3C6) Alexa Fluor® 488 (green) in 5% FBS overnight at 4°C, followed by Phalloidin Alexa Fluor® 647 (red) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Compare all formats See Alexa Fluor® 488 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

674006

100 µg

382 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Nanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human recombinant protein expressed in bacteria
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2571952 (BioLegend Cat. No. 674006)

Antigen Details

Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.
Function: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction: Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676.
2. Torres-Padilla ME, et al. 2014. Development 141:2173.
3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741.
4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207.
5. Kim J, et al. 2008. Cell 132:1049.
6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081.
7. Yu J, et al. 2007. Science 318:1917.
Gene ID: 79923 View all products for this Gene ID
UniProt: View information about Nanog on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Immunocytochemistry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Nanog Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nanog	23D2-3C6	WB,ICFC,ICC
Alexa Fluor® 594 anti-Nanog	23D2-3C6	ICC
Alexa Fluor® 488 anti-Nanog	23D2-3C6	ICC
Alexa Fluor® 647 anti-Nanog	23D2-3C6	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.