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Alexa Fluor® 488 anti-RBFOX1 (A2BP1) Antibody

Pricing & Availability
Clone
A18069B (See other available formats)
Regulatory Status
RUO
Other Names
RBFox1, A2BP1, RNA Binding fox-1 Homolog 1, Hexaribonucleotide Binding Protein 1, FOX1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A18069B_A488_RBFOX1_Antibody_1_041020
IHC staining of Alexa Fluor® 488 anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18069B_A488_RBFOX1_Antibody_1_041020
    IHC staining of Alexa Fluor® 488 anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18069B_A488_RBFOX1_Antibody_1_041020
    IHC staining of Alexa Fluor® 488 anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928502), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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862709 25 µg 153 CHF
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862710 100 µg 323 CHF
 
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Description

Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
RRID
AB_2861087 (BioLegend Cat. No. 862709)
AB_2861087 (BioLegend Cat. No. 862710)

Antigen Details

Structure
RBFOX1 is a 416 amino acid protein with an apparent molecular mass of ~ 45 kD.
Distribution

Tissue Distribution: brain and heart.
Cellular Distribution: nucleus, golgi apparatus, and cytosol.

Function
RBFOX1 is an RNA binding protein that modulates mRNA alternative splicing.
Interaction
RNA, ataxin-2
Cell Type
Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Protein Synthesis, Transcription Factors
Antigen References
  1. Wang ET, et al. 2016. J. Neurosci. 36(45):11418
  2. Duan W, et al. 2016. Mol Neurobiol. 53(3):1637
  3. Kuroyanagi H, 2009. Cell Mol Life Sci. 66(24):3895
Gene ID
54715 View all products for this Gene ID 268859 View all products for this Gene ID 302920 View all products for this Gene ID
UniProt
View information about RBFOX1 on UniProt.org
Go To Top Version: 1    Revision Date: 04.10.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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