- Clone
- W19258A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Avi-Tag, AviTag, Avi epitope Tag
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W19258A_A594_AviTag_Antibody_051823 W19258A_A594_AviTag_Antibody_051823](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W19258A_A594_AviTag_Antibody_051823.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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CHO cells untransfected (left panel) or transfected (right panel) with an expression vector for VEGF-165-Avi-tag were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with Alexa Fluor® 594 anti-Avi-tag (clone W19258A) overnight at 4°C. Nuclei and actin filaments were counterstained with DAPI (Cat. No. 422801) and Flash Phalloidin™ Green 488 (Cat. No. 424201). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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606758 | 100 µg | 370 CHF |
Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli. Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.
- Additional Product Notes
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ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100
- RRID
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AB_3068041 (BioLegend Cat. No. 606758)
Antigen Details
- Structure
- GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
- Distribution
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Depends on the subcellular location of the tagged protein
- Biology Area
- Cell Biology, Protein Purification, Protein Trafficking and Clearance
- Gene ID
- NA
- UniProt
- View information about Avi-tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Avi-tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Avi-tag | W19258A | WB,ICC,ICFC,IP |
Alexa Fluor® 488 anti-Avi-tag | W19258A | ICFC,ICC |
Alexa Fluor® 594 anti-Avi-tag | W19258A | ICC |
Alexa Fluor® 647 anti-Avi-tag | W19258A | ICFC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Avi-tag
Whole cell extracts (15 µg total protein/lane) from HEL cell... Whole cell extracts (300 µg) total protein prepared from unt... CHO cells untransfected or transfected with an expression ve... CHO cells transfected with an expression vector for VEGF-165... -
Alexa Fluor® 488 anti-Avi-tag
CHO cells untransfected (negative control, left panel) or ex... CHO cells untransfected (left panel) or transfected (right p... -
Alexa Fluor® 594 anti-Avi-tag
CHO cells untransfected (left panel) or transfected (right p... -
Alexa Fluor® 647 anti-Avi-tag
CHO cells untransfected (negative control, left panel) or ex... CHO cells untransfected (left panel) or transfected (right p...
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