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Alexa Fluor® 594 anti-human ACE2 Antibody

Pricing & Availability
Clone
A20069I (See other available formats)
Regulatory Status
RUO
Other Names
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACE-2, ACEH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20069I_AF594_ACE2_Antibody_042122
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL Alexa Fluor® 594 anti-human ACE2 (clone A20069I) (red) and 10 µg/mL Alexa Fluor® 647 anti-human CD79a (clone HM47, green) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A20069I_AF594_ACE2_Antibody_042122
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL Alexa Fluor® 594 anti-human ACE2 (clone A20069I) (red) and 10 µg/mL Alexa Fluor® 647 anti-human CD79a (clone HM47, green) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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375805 25 µg 154 CHF
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375806 100 µg 352 CHF
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Description

Angiotensin-converting enzyme 2 (ACE2) is a type one transmembrane protein, which shares approximately 60% homology with ACE. The predicted human ACE2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction. Recently, ACE2 has been shown to be a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Based on recent single-cell RNA sequencing (scRNA-Seq) database, high ACE2 RNA expression was identified in type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, and endothelium and vascular smooth muscle cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein cover extracellular domain of ACE2 (Gln 18-Ser740)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2927820 (BioLegend Cat. No. 375805)
AB_2927820 (BioLegend Cat. No. 375806)

Antigen Details

Structure
Type one transmemberance protein. Protein sequence consistent extracellular domain (Gln 18-Ser740 ) contain cytalitic domain, transmembrane domain (AA741-AA761) and cytoplasmic domain (AA762-AA805).
Distribution

Type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, endothelium and vascular smooth muscle cells

Function
Catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction
Interaction
Angiotensin II, ADAM17, TMPRSS2
Ligand/Receptor
Functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus)
Cell Type
Monocytes
Biology Area
Angiogenesis, COVID-19, Immunology
Molecular Family
Enzymes and Regulators
Antigen References
  1. South AM, et al. 2020. Nat Rev Nephrol. 16:305-7.
  2. Hoffmann M, et al. 2020. Cell. 181:271-80.
  3. Sungnak W, et al. 2020. Nat Med. 26:681-7.
  4. Li W, et al. 2003. Nature. 426:450-4.
  5. Heurich A, et al. 2014. J Virol. 88:1293-30.
  6. Hamming I, et al. 2004. J Pathol. 203:631-7.
Gene ID
59272 View all products for this Gene ID
UniProt
View information about ACE2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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