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Alexa Fluor® 594 anti-human CD79a (Igα) Antibody

Pricing & Availability
Clone
HM47 (See other available formats)
Regulatory Status
RUO
Workshop
V cB017
Other Names
Mb-1, Iga, CD79
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HM47_A594_CD79a_Antibody_052218
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10 mM, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-CD8a (clone C8/144B, green) antibody and Alexa Fluor® 594 anti-CD79a (clone HM47, red) antibody over night at 4°C. The nuclei were counter stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • HM47_A594_CD79a_Antibody_052218
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10 mM, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-CD8a (clone C8/144B, green) antibody and Alexa Fluor® 594 anti-CD79a (clone HM47, red) antibody over night at 4°C. The nuclei were counter stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 594 spectral data
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333513 25 µg 159 CHF
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333514 100 µg 370 CHF
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Description

CD79a is a 47 kD type I integral membrane protein, also known as mb-1 or Iga. It is a member of the Ig superfamily and disulphide-associated with CD79b (B29). The interaction of CD79a/CD79b heterodimer with B cell suface Ig forms B cell antigen receptor complex. CD79a is expressed in B cells from early pre-B to plasma cell stage. It has been shown that CD79a is also weakly expressed in some precursors of T- and myeloid cells. CD79 mediates the transport of IgM to B cell surface and transduces signals initiated by BCR aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2734328 (BioLegend Cat. No. 333513)
AB_2734328 (BioLegend Cat. No. 333514)

Antigen Details

Structure
47 kD, type I integral protein, Ig superfamily, CD79a/CD79b heterodimer interacts with B cell membrane Ig.
Distribution

B cells (from early pre-B to plasma cell stage), some T and myeloid cell precursors

Function
In association with CD79b and B cell membrane Ig to form B cell antigen receptor complex
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Astsaturov IA, et al. 1996. Leukemia 10:769
3. Mson DY, et al. 1995 Blood 86:1453
4. Hashimoto M, et al. 2002. J. Pathol. 197:341

Gene ID
973 View all products for this Gene ID
UniProt
View information about CD79a on UniProt.org
Go To Top Version: 1    Revision Date: 06.25.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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