IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse thalamus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Methyl CpG binding protein (MECP2), also known as autism susceptibility to X-linked 2 (AUTSX3), is a nuclear protein crucial for normal neuronal function that binds to methylated DNA, forming complexes with histone deacteylases and turning off gene expression. MECP2 expression is an X-linked trait. Loss of MECP2 expression causes Rett Syndrome (also known as RTS, RTT, or RS) a neurological disorder which causes decelerated growth of hands, feet, and the head.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
Follow Us