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Alexa Fluor® 594 anti-mouse CD5 Antibody

Pricing & Availability
Clone
53-7.3 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype
Rat IgG2a, κ
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Product Citations
publications
53-73_A594_CD5_Antibody_1_060319
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD5 (clone 53-7.3) Alexa Fluor® 594 (red), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (green), and 5 µg/ml of CD45R/B220 (clone RA3-6B2) Brilliant Violet™ 421 (blue) overnight at 4°C. The image was captured with a 10X objective.
  • 53-73_A594_CD5_Antibody_1_060319
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD5 (clone 53-7.3) Alexa Fluor® 594 (red), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (green), and 5 µg/ml of CD45R/B220 (clone RA3-6B2) Brilliant Violet™ 421 (blue) overnight at 4°C. The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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100632 100 µg 159 CHF
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Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
  3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
RRID
AB_2566434 (BioLegend Cat. No. 100632)

Antigen Details

Structure
Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution

Thymocytes, T cells, B-1 cells

Function
Negative regulator of T-B cell interaction
Ligand/Receptor
CD72, gp35-37
Cell Type
B cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.

Gene ID
12507 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org
Go To Top Version: 1    Revision Date: 10.30.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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