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Alexa Fluor® 594 anti-mouse IgG2b Antibody

Pricing & Availability
Clone
RMG2b-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Rat IgG1, κ
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Product Citations
publications
RMG2b-1_A594_IgG2b_Antibody_031618
C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • RMG2b-1_A594_IgG2b_Antibody_031618
    C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • RMG2b-1_A594_IgG2b_Antibody_2_062123
    IHC staining of Alexa Fluor® 594 anti-mouse IgG2b on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 2.5 µg/mL of purified mouse anti-CD45RA (Cat. No. 304102) at 4°C. This was followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG2b (clone RMG2b-1) at 10 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability Save
406709 25 µg 106 CHF
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406710 100 µg 200 CHF
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Description

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2728428 (BioLegend Cat. No. 406709)
AB_2728428 (BioLegend Cat. No. 406710)

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Go To Top Version: 2    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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