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- Clone
- SA024B4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly6/PLAUR domain containing 8
- Isotype
- Mouse IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/mL of Alexa Fluor® 594 Lypd8 (clone SA024B4) overnight at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 152905 | 25 µg | 159 CHF | ||||
| 152906 | 100 µg | 382 CHF | ||||
Ly6/PLAUR domain containing 8 (Lypd8) is a 100-120 kD, GPI-anchored glycoprotein, a member of the Ly6/PLAUR Family. Lypd8 is highly expressed on epithelial cells from intestinal lumen, where prevents bacterial invasion inhibiting bacterial motility by binding to flagella.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse colon tissue
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration of 2.5 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
This clone recognizes Lypd8 from BALB/c and C57BL/6 mice.
- RRID
-
AB_2721386 (BioLegend Cat. No. 152905)
AB_2721386 (BioLegend Cat. No. 152906)
Antigen Details
- Structure
- GPI-anchored, glycoprotein, 100-120 kD.
- Distribution
-
Epitelials cells in cecum and large intestine.
- Function
- Inhibits bacterial motility by binding to flagella.
- Cell Type
- Epithelial cells
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- 70163 View all products for this Gene ID
- UniProt
- View information about Lypd8 on UniProt.org
Related FAQs
Other Formats
View All Lypd8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Lypd8 | SA024B4 | IHC-F,FC,IHC-P |
| Alexa Fluor® 594 anti-mouse Lypd8 | SA024B4 | IHC-F |
| Alexa Fluor® 647 anti-mouse Lypd8 | SA024B4 | IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Lypd8
C57BL/6 mouse frozen colon section was fixed with 4% parafor... -
Alexa Fluor® 594 anti-mouse Lypd8
C57BL/6 mouse frozen colon section was fixed with 4% parafor... -
Alexa Fluor® 647 anti-mouse Lypd8
C57BL/6 mouse frozen colon section was fixed with 4% parafor...
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