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Alexa Fluor® 594 anti-Nestin Antibody

Pricing & Availability
Clone
10C2 (See other available formats)
Regulatory Status
RUO
Other Names
NES
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_10C2_AF594_Nestin_Antibody_IF_072414_large
HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml of anti-human Nestin (clone 10C2) Alexa Fluor® 594 overnight, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • A_10C2_AF594_Nestin_Antibody_IF_072414_large
    HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml of anti-human Nestin (clone 10C2) Alexa Fluor® 594 overnight, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • B_10C2_AF594_Nestin_Antibody_IHC-P_100516_large
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween20 twice for five minutes , permeabilized with 0.5% Trito-X 100 for ten minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of purified anti-human CD133 (clone7) at 4°C overnight. On the next day, tissue was washed with PBS twice
Compare all formats See Alexa Fluor® 594 spectral data
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656804 100 µg 347 CHF
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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
RRID
AB_2563509 (BioLegend Cat. No. 656804)

Antigen Details

Structure
1621 amino acids with predicted molecular weight of 200-220 kD
Distribution

Cytosol

Function
Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Antigen References

1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.

Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org
Go To Top Version: 2    Revision Date: 10.05.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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