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Alexa Fluor® 594 anti-NR1D2 Antibody

Pricing & Availability
Clone
W15136A (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear receptor subfamily 1 group D member 2, BD73, V-erbA-related protein 1-related (EAR-1r), Hs.37288, HZF2, Rev-erb alpha-related receptor (RVR), Rev-erb-beta
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W15136A_A594_NR1D2_Antibody_110317
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with either (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 Rat IgG1, or Alexa Fluor® 594 anti-NR1D2 antibody (red) at (B) 1: 100 (5 µgg/ml), (C) 1: 250 (2 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). Actin filaments were labeled with Flash Phalloidin™ Green 488 (green). The image was captured with a 60X objective.
  • W15136A_A594_NR1D2_Antibody_110317
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with either (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 Rat IgG1, or Alexa Fluor® 594 anti-NR1D2 antibody (red) at (B) 1: 100 (5 µgg/ml), (C) 1: 250 (2 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). Actin filaments were labeled with Flash Phalloidin™ Green 488 (green). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
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689403 25 µg 159 CHF
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Description

Nuclear receptor subfamily 1, group D, member 2 (NR1D2), also known as Rev-ErbA β, is a member of the nuclear hormone receptor family. This protein can modulate gene expression by directly binding to DNA response elements within the promotor of its target genes as either monomers or homodimers. NR1D2 is well known to function as a transcriptional repressor. By complexing with heme, NR1D2 coordinates circadian rhythm and controls lipid metabolism. NR1D2 can directly repress the expression of core clock components ARNTL/BMAL1 and CLOCK, to coordinate circadian rhythm. In addition, NR1D2 can regulate lipid and energy homeostasis in skeletal muscles via repression of lipid metabolism and myogenesis-related genes such as CD36, FABP, SCD1 and MSTN. It is also involved in the regulation of hepatic lipid metabolism through the suppression of APOC3 expression. Not only as a repressor, NR1D2 also acts as a transcriptional activator; it can activate the transcription of the sterol regulatory element-binding protein 1 (STEBF1) and the inflammatory mediator IL-6 in skeletal muscle. Alternatively spliced transcript variants of NR1D2 gene have been described.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human NR1D2 recombinant protein (196-399 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2721394 (BioLegend Cat. No. 689403)

Antigen Details

Structure
579 amino acids, predicted molecular weight of 65 kD. Contains two N-terminal zinc finger domains responsible for DNA binding.
Distribution

Nucleus.

Function
NR1D2 belongs to the nuclear hormone receptor family and acts as a transcriptional repressor. NR1D2 participates in the regulation of circadian rhythms and energy homeostasis.
Interaction
Forms a DNA-binding homodimer. Interacts with NCOA5, KAT5, HDAC1, and ZNHIT1.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References

1. Cho H, et al. 2012. Nature 485:123.
2. Gupta N, Ragsdale SW. 2011. J. Biol. Chem. 286:4392.
3. Bois-Joyeux B, et al. 2000. DNA Cell Biol. 19:589-99.
4. Burke L, et al. 1996. Nucleic Acids Res. 24:3481.
5. Giambiagi N, et al. 1995. Biochem. Mol. Biol. Int. 37:1091.
6. Bonnelye E, et al. Cell Growth Differ. 5:1357.

Gene ID
9975 View all products for this Gene ID
UniProt
View information about NR1D2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.06.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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