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Alexa Fluor® 594 anti-NSE Antibody

Pricing & Availability
Clone
NSE-P1 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Isotype
Mouse IgG1, κ
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Product Citations
publications
NSE-P1_A594_NSE_Antibody_1_120617
ICC staining of Alexa Fluor® 594 anti-NSE antibody (clone NSE-P1) on MCF7 cells. The cells were fixed with methonal, permeabilized with a buffer containing 0.25% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 20 µm.
  • NSE-P1_A594_NSE_Antibody_1_120617
    ICC staining of Alexa Fluor® 594 anti-NSE antibody (clone NSE-P1) on MCF7 cells. The cells were fixed with methonal, permeabilized with a buffer containing 0.25% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 20 µm.
  • NSE-P1_A594_NSE_Antibody_2_120617
    IHC staining of Alexa Fluor® 594 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse (A) and rat (B) brain tissues. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.) (Cat. No. 928502), tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. The images were captured with a 40X objective. Scale bar: 20 µm.
Compare all formats See Alexa Fluor® 594 spectral data
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804902 25 µg 124 CHF
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Description

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The reactivity of Alexa Fluor® 647 and Alexa Fluor® 488 anti-NSE antibody was verified in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA2 and immunohistochemistry1,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
  2. Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
  3. Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
  4. Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)
RRID
AB_2721395 (BioLegend Cat. No. 804902)

Antigen Details

Structure
Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Nervous and musculoskeletal systems.
Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type
Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Enzymes and Regulators, Phospho-Proteins
Antigen References
  1. Muoio B, et al. 2017. Int J Biol Markers. 10.5301/ijbm.5000286.
  2. Huang L, et al. 2017. Oncotarget. 8(38): 64358.
  3. Isgro MA, et al. 2015. Adv Exp Med Biol. 867:125.
  4. Zaheer S, et al. 2013. Ann Indian Acad Neurol. 16(4):504.
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.14.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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