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Alexa Fluor® 594 anti-SATB1 Antibody

Pricing & Availability
Clone
O96C6 (See other available formats)
Regulatory Status
RUO
Other Names
Special AT-rich sequence-binding protein 1, SATB homeobox 1, DNA-binding protein SATB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O96C6_AF594_SATB1_IF_032318
Jurkat cells were fixed with 1% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-SATB1 antibody (clone O96C6, red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue).
  • O96C6_AF594_SATB1_IF_032318
    Jurkat cells were fixed with 1% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-SATB1 antibody (clone O96C6, red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue).
See Alexa Fluor® 594 spectral data
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676104 100 µg 370 CHF
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Description

SATB1 is a global regulator that selectively tethers multiple genomic loci and recruits chromatin-remodeling enzymes (e.g. ACF, ISWI, HDAC1 and histone acetyltransferase) to control chromatin structure and gene expression. SATB1 also recruits corepressors or coactivators to promoters. These two opposing interactions, however, are determined by whether or not the SATB1 is phosphorylated by protein kinase C. Unphosphorylated and phosphorylated SATB1 preferably binds to PCAF and HDAC1, respectively. SATB1 also forms a regulatory complex with the promyelocytic leukaemia (PML) protein to organize the major histocompatibility complex (MHC) class I locus into distinct chromatin-loop architecture and mediate the expression of a subset of MHC class I genes. Upon Th2 cell activation, SATB1 expression is induced to compact chromatin into dense-loop structure and to prompt the expression of IL-4, IL-5, IL-13 and c-Maf. SATB1 is also reported as being crucial for the growth and metastasis of breast cancer and is a potential prognostic marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human SATB1 (NP_002962) produced in HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2566180 (BioLegend Cat. No. 676104)

Antigen Details

Structure
Homodimer. Canonical sequence contains 763 amino acids, predicted molecular weight of 86 kD. Isoform contains 795 amino acids, predicted molecular weight of 89 kD.
Distribution

Nucleus matrix. Nucleus. Promyelocytic leukemia nuclear bodies (SUMOylated).

Function
SATB1 recruits chromatin remodeling factors to regulate chromatin-loop structure and gene transcription. It is an important silencing factor for initiating the Xist RNA-mediated X inactivation in mammals.
Interaction
CUX1, CtBP1, PCAF, SUMOylated PML, HDAC1 and HIV-1 Tat, DYNLT2, POLR2J2, EP300, special AT-rich DNA sequences (consensus SATB1-binding sequence, CSBS).
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Agrelo R, et al. 2009. Dev. Cell. 16:507.
2. Han HJ, et al. 2008. Nature. 452:187.
3. Cai S, et al. 2006. Nature Genet. 38:1278.
4. Pavan Kumar, P, et al. 2006. Mol. Cell. 22:231.
5. Fujii Y, et al. 2003. Microbiol. Immunol. 47:803.
6. Yasui, D, et al. 2002. Nature. 419:641.

Gene ID
6304 View all products for this Gene ID
UniProt
View information about SATB1 on UniProt.org
Go To Top Version: 2    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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