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Alexa Fluor® 594 anti-STAT1 Phospho (Ser727) Antibody

Pricing & Availability
Clone
A15158B (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15158B_A594_STAT1_Phospho_Antibody_1_060718
Two aliquots of HeLa cells in suspension, treated with 200 ng/mL nocodazole for 24 hours (left) or left un-treated (right) were adhered on poly-lysine pre-coated slides. Then they were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 0.5 µg/ml Alexa Fluor® 594 anti-STAT1 Phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • A15158B_A594_STAT1_Phospho_Antibody_1_060718
    Two aliquots of HeLa cells in suspension, treated with 200 ng/mL nocodazole for 24 hours (left) or left un-treated (right) were adhered on poly-lysine pre-coated slides. Then they were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 0.5 µg/ml Alexa Fluor® 594 anti-STAT1 Phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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686405 25 µg 159 CHF
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT1 peptide phosphorylated at Ser 727.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.25 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Product Citations
  1. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
RRID
AB_2617021 (BioLegend Cat. No. 686405)

Antigen Details

Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Translocates to the nucleus when phosphorylated.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619.

Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho Ser727 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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