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Alexa Fluor® 594 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Alexa Fluor® 594, Streptavidin-AF594, SAV
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Product Citations
publications
SAV_A594_Streptavidin_Antibody_1_121819
Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 20X objective.
  • SAV_A594_Streptavidin_Antibody_1_121819
    Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 20X objective.
  • SAV_A594_Streptavidin_Antibody_2_121819
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data
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405240 100 µg 265 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 594 is useful for detecting biotinylated antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F - Verified
IHC-P
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 594 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Streptavidin-Alexa Fluor® 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 594 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010. PubMed
  3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
  4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
  8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
  9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
Product Citations
  1. Cheng WH, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  3. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  4. Thuault S, et al. 2020. Sci Rep. 10:6787. PubMed
  5. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  6. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  7. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  8. Soukupova M, et al. 2021. Mol Ther Methods Clin Dev. 21:399. PubMed
  9. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  10. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed

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Go To Top Version: 3    Revision Date: 12.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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