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Alexa Fluor® 647 anti-c-Fos Antibody

Pricing & Availability
Clone
7D6-A9-D4 (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_7D6-A9-D4_A647_c-Fos_Antibody_010824
HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A)(Cat. No. 423301) for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-c-Fos (clone 7D6-A9-D4) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400130).
  • A_7D6-A9-D4_A647_c-Fos_Antibody_010824
    HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A)(Cat. No. 423301) for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-c-Fos (clone 7D6-A9-D4) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400130).
  • B_7D6-A9-D4_A647_c-Fos_Antibody_ICC_010824
    HeLa cells untreated (top panels), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (bottom panels) were fixed and permeabilized with 2% PFA and ice-cold methanol, and then intracellularly stained with Alexa Fluor® 647 anti-c-Fos (clone 7D6-A9-D4)(right panels) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400130) (left panels). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). Scale bar 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
600755 25 µg 195 CHF
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600756 100 µg 475 CHF
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Description

The c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. C-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human FOS protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal.

 

For ICFC, we recommend fixation/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal.

RRID
AB_3097469 (BioLegend Cat. No. 600755)
AB_3097469 (BioLegend Cat. No. 600756)

Antigen Details

Structure
c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.
Distribution

Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes

Function
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
Interaction
Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Dony C and Gruss P. 1987. Nature. 328:711-4.
  2. Grigoriadis AE, et al. 1994. Science. 266:443-8.
  3. Glover JN and Harrison SC. 1995. Nature. 373:257-61.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org
Go To Top Version: 1    Revision Date: 01.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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