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Alexa Fluor® 647 anti-EGFR Antibody

Pricing & Availability
Clone
A19002A (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA, Proto-oncogene c-ErbB-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A19002A_AF647_EGFR_Antibody_1_090920.png
A-431 cells were stained intracellularly with anti-EGFR (clone A19002A) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • A19002A_AF647_EGFR_Antibody_1_090920.png
    A-431 cells were stained intracellularly with anti-EGFR (clone A19002A) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • A19002A_AF647_EGFR_Antibody_2_090920.png
    Formalin-fixed paraffin-embedded human breast cancer tissue slices were deparaffinized and rehydrated. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0 M, pH 7.4) at 95°C for 40 minutes, washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the slices were stained with 5 µg/mL anti-EGFR (clone A19002A) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A19002A_AF647_EGFR_Antibody_2_090920.png
    Formalin-fixed paraffin-embedded human placenta tissue slices were deparaffinized and rehydrated. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0 M, pH 7.4) at 95°C for 40 minutes, washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the slices were stained with 5 µg/mL anti-EGFR (clone A19002A) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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933903 25 tests 112 CHF
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933904 100 tests 282 CHF
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Description

Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human EGFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This clone was tested for ICC using 4% PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, methanol enabled superior staining.

This clone is predicted to recognize isoform I based off of complete sequence homology of the immunizing peptide and the corresponding region of the isoform.

 

RRID
AB_2876751 (BioLegend Cat. No. 933903)
AB_2876751 (BioLegend Cat. No. 933904)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors
Antigen References
  1. Hunter T and Cooper JA. 1981. Cell. 24:741.
  2. Gill GN and Lazar CS. 1981. Nature. 293:305.
  3. Reynolds FH, et al. 1981. Nature. 292:259
  4. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Other Formats

View All EGFR Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-EGFR A19002A WB,ICC,IHC-P,ICFC,IP
Alexa Fluor® 647 anti-EGFR A19002A ICFC,IHC-P
PerCP/Cyanine5.5 anti-EGFR A19002A ICFC
Go To Top Version: 1    Revision Date: 09.09.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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