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Alexa Fluor® 647 anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
J099B12_A647_His_Antibody_100617
Hela cells transfected with multi-tag plasmid were fixed with cold methanol for 15 minutes at -20°C and then blocked with 5% FBS for 60 minutes. Cells were stained with either (A) 1:500 (1 µg/ml) Alexa Fluor® 594 mouse IgG1, κ isotype control antibody (Cat no. 400135), or (B, C) 1:500 (1 µg/ml), (D, E) 1:1000 (0.5 µg/ml), (F) 1:2500 (0.2 µg/ml) Alexa Fluor® 647 anti-His antibody (red), and incubated overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (S): (A) 1/1.5, (B, D, F) 1/30, (C) 1/150, (E) 1/70.
  • J099B12_A647_His_Antibody_100617
    Hela cells transfected with multi-tag plasmid were fixed with cold methanol for 15 minutes at -20°C and then blocked with 5% FBS for 60 minutes. Cells were stained with either (A) 1:500 (1 µg/ml) Alexa Fluor® 594 mouse IgG1, κ isotype control antibody (Cat no. 400135), or (B, C) 1:500 (1 µg/ml), (D, E) 1:1000 (0.5 µg/ml), (F) 1:2500 (0.2 µg/ml) Alexa Fluor® 647 anti-His antibody (red), and incubated overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (S): (A) 1/1.5, (B, D, F) 1/30, (C) 1/150, (E) 1/70.
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652513 25 µg 159 CHF
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution 1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  2. Yusuf B, et al. 2021. Sci Rep. 11:3614. PubMed
  3. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
RRID
AB_2716153 (BioLegend Cat. No. 652513)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
Go To Top Version: 2    Revision Date: 09.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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