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Alexa Fluor® 647 anti-human CD66a/b/c/e Antibody

Pricing & Availability
Clone
5B2 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1,5,6,8
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5B2_AF647_CD66a-b-c-e_Antibody_1_092320.png
Human colorectal adenocarcinoma cell line were stained with CD66a/b/c/e (clone 5B2) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ (clone MOPC-21) Alexa Fluor® 647 isotype control (open histogram).
  • 5B2_AF647_CD66a-b-c-e_Antibody_1_092320.png
    Human colorectal adenocarcinoma cell line were stained with CD66a/b/c/e (clone 5B2) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ (clone MOPC-21) Alexa Fluor® 647 isotype control (open histogram).
  • 5B2_AF647_CD66a-b-c-e_Antibody_2_092320.png
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD66a/b/c/e (clone 5B2) Alexa Fluor® 647 (red) and anti-Vimentin (clone O91D3) Alexa Fluor® 594 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with the MetaMorph® software.
  • 5B2_AF647_CD66a-b-c-e_Antibody_3_092320.png
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD66a/b/c/e (clone 5B2) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
398905 25 tests 141 CHF
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398906 100 tests 340 CHF
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Description

The 5B2 monoclonal antibody reacts with the antigen epitopes shared by CD66a, b, c and e, which are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neisseria, Moraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
RRID
AB_2888720 (BioLegend Cat. No. 398905)
AB_2888720 (BioLegend Cat. No. 398906)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Ligand/Receptor
CD66a-e, CD62E, galectins
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
  2. Chen T, et al. 2001. J Biol Chem. 276:17413.
  3. Wang Z, et al. 2016. Mol Clin Oncol. 5:731.
  4. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID
1088 View all products for this Gene ID 634 View all products for this Gene ID 4680 View all products for this Gene ID 1048 View all products for this Gene ID
UniProt
View information about CD66a/b/c/e on UniProt.org
Go To Top Version: 1    Revision Date: 09.23.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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