Alexa Fluor 647 anti-human IgE Antibody anti-IgE

Pricing & Availability

Clone: MHE-18 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHE-18_A647_IgE_Antibody_1_050517 — Overnight cultured human peripheral blood mononuclear cells were stained with CD193 PE and anti-human IgE (clone MHE-18) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 (bottom). Data shown was gated on lymphocyte population.

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325513

25 tests

165 CHF

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325514

100 tests

347 CHF

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Description

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
RRID: AB_2687179 (BioLegend Cat. No. 325513)
AB_2687179 (BioLegend Cat. No. 325514)

Antigen Details

Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497 View all products for this Gene ID
UniProt: View information about IgE on UniProt.org

Related Products

Description	Clone	Applications

Other Formats

View All IgE Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human IgE	MHE-18	FC,ELISA,IHC
Biotin anti-human IgE	MHE-18	FC,ELISA,IHC
PE anti-human IgE	MHE-18	FC
APC anti-human IgE	MHE-18	FC
PE/Cyanine7 anti-human IgE	MHE-18	FC
PerCP/Cyanine5.5 anti-human IgE	MHE-18	FC
Alexa Fluor® 647 anti-human IgE	MHE-18	FC
APC/Fire™ 750 anti-human IgE	MHE-18	FC
TotalSeq™-A0868 anti-human IgE	MHE-18	PG
APC/Cyanine7 anti-human IgE	MHE-18	FC
TotalSeq™-C0868 anti-human IgE	MHE-18	PG
TotalSeq™-B0868 anti-human IgE	MHE-18	PG

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Go To Top Version: 0 Revision Date: 05.08.2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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