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Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
281-2_AF647_CD138_Antibody_FC_1_092515
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data shown is gated on the lymphoid population.
  • 281-2_AF647_CD138_Antibody_FC_1_092515
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data shown is gated on the lymphoid population.
  • 281-2_AF647_CD138_Antibody_FC_2_092515
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
142525 25 µg 159 CHF
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142526 100 µg 393 CHF
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  2. van Attekum MHA, et al. 2017. Oncogene. 3651:36. PubMed
  3. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  4. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  5. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  6. Werner A, et al. 2021. iScience. 24:103076. PubMed
RRID
AB_2566238 (BioLegend Cat. No. 142525)
AB_2566238 (BioLegend Cat. No. 142526)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD138 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD138 (Syndecan-1) 281-2 FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1) 281-2 FC
APC anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) 281-2 FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1) 281-2 FC
Biotin anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1) 281-2 FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Go To Top Version: 1    Revision Date: 09.29.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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