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- Clone
- 9B1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GPR 9-6, CDw199, Chemokine Receptor 9
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 thymocytes stained with 9B1 Alexa Fluor® 647
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 129709 | 25 µg | 147 CHF | ||||
| 129710 | 100 µg | 347 CHF | ||||
CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Product Citations
-
- RRID
-
AB_2073247 (BioLegend Cat. No. 129709)
AB_2073247 (BioLegend Cat. No. 129710)
Antigen Details
- Structure
- A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
- Distribution
- CD4+CD8+ thymocytes, γ/δ T cells
- Interaction
- CCL25
- Ligand/Receptor
- CCL25
- Cell Type
- T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Zaballos A, et al. 1999. J. Immunol. 162:5671
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73 - Gene ID
- 12769 View all products for this Gene ID
- UniProt
- View information about CD199 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Other Formats
View All CD199 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse CD199 (CCR9) | 9B1 | FC |
| PE anti-mouse CD199 (CCR9) | 9B1 | FC |
| Alexa Fluor® 647 anti-mouse CD199 (CCR9) | 9B1 | FC |
| Ultra-LEAF™ Purified anti-mouse CD199 (CCR9) | 9B1 | FC,FA |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD199 (CCR9)
C57BL/6 thymocytes stained with 9B1 FITC -
PE anti-mouse CD199 (CCR9)
C57BL/6 thymocytes stained with 9B1 PE -
Alexa Fluor® 647 anti-mouse CD199 (CCR9)
C57BL/6 thymocytes stained with 9B1 Alexa Fluor® 647 -
Ultra-LEAF™ Purified anti-mouse CD199 (CCR9)
C57BL/6 thymocytes stained with Ultra-LEAF™ Purified 9B1, fo...
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