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Alexa Fluor® 647 anti-mouse CD22 Antibody

Pricing & Availability
Clone
OX-97 (See other available formats)
Regulatory Status
RUO
Other Names
Lyb-8, BL-CAM
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
OX-97_A647_081908.jpg
C57BL/6 splenocytes stained with OX-97 Alexa Fluor® 647
  • OX-97_A647_081908.jpg
    C57BL/6 splenocytes stained with OX-97 Alexa Fluor® 647
  • OX-97_A647_CD22_Antibody_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD22 (clone OX-97) Alexa Fluor® 647 (red) and CD3 (clone 17A2) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective.
  • OX-97_A647_CD22_Antibody_3_091721.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD22 Antibody (clone OX-97) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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126107 25 µg 124 CHF
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126108 100 µg 265 CHF
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Description

CD22 is a member of the sialoadhesion family.CD22 is expressed on the surface of a subpopulation of mature B lymphocytes and may modulate signaling through the B cell antigen receptor. The OX-97 monoclonal antibody recognizes the second domain of the CD22.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed
  2. Cao A, et al. 2018. Nat Commun. 9:3288. PubMed
  3. Smith LK, et al. 2021. Elife. 10:. PubMed
  4. Wen T, et al. 2012. J Immunol. 188:1075. PubMed
RRID
AB_2074573 (BioLegend Cat. No. 126107)
AB_2074573 (BioLegend Cat. No. 126108)

Antigen Details

Structure
130-150 kd type 1 transmembrane glycoprotein. It is a member of the Siglec family and Ig superfamily.
Distribution

CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells.

Function
B cell signaling and adhesion.
Interaction
Associates with SHP-1, Syk, Lck, Lyn and others.
Cell Type
B cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Symington FW et al. 1982. Immunogenetics 16:381
2. Erickson LD et al. 1996. Int Immunol 8:1121
3. Doody GM et al. 1995. Science 269:242

Gene ID
12483 View all products for this Gene ID
UniProt
View information about CD22 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09.17.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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