Alexa Fluor 647 anti-mouse FR4 Folate Receptor 4 Antibody anti-FR4

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Clone: 12A5 (See other available formats)
Regulatory Status: RUO
Other Names: Folate Receptor 4, FBP, FR δ
Isotype: Rat IgG1, κ
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Product Citations: publications

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and 12A5 Alexa Fluor® 647

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125009

25 µg

147 CHF

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Description

Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_1134201 (BioLegend Cat. No. 125009)

Antigen Details

Structure: 28 kd protein consisting of 244 amino acids. Heavily glycosylated
Distribution: Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
Function: Mediate calcium-independent homophlic cell-cell adhesion
Ligand/Receptor: Folic acid and its derivative
Cell Type: Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Yamaguchi T et al 2007. Immunity 27:145
2. Spiegelstein O et al. 2000 Gene 258:117
Gene ID: 64931 View all products for this Gene ID
UniProt: View information about FR4 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All FR4 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse FR4 (Folate Receptor 4)	12A5	FC
PE anti-mouse FR4 (Folate Receptor 4)	12A5	FC
Alexa Fluor® 647 anti-mouse FR4 (Folate Receptor 4)	12A5	FC
PE/Cyanine7 anti-mouse FR4 (Folate Receptor 4)	12A5	FC
PerCP/Cyanine5.5 anti-mouse FR4 (Folate Receptor 4)	12A5	FC
PE/Dazzle™ 594 anti-mouse FR4 (Folate Receptor 4)	12A5	FC
APC/Fire™ 750 anti-mouse FR4 (Folate Receptor 4)	12A5	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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