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Alexa Fluor® 647 anti-mouse H-2D b Antibody

Pricing & Availability
Clone
KH95 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2b, κ
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Product Citations
publications
KH95_Alx647_020708
C57BL/6 mouse splenocytes stained with KH95 Alexa Fluor® 647
  • KH95_Alx647_020708
    C57BL/6 mouse splenocytes stained with KH95 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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111512 100 µg 229 CHF
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Description

The KH95 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10 mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
  2. Shao H, et al. 2005. J. Immunol. 175:1851.
  3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
  4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
  5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
  6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed
Product Citations
  1. Rowe RG, et al. 2019. J Exp Med. 527:216. PubMed
  2. Milstein O, et al. 2011. Blood. 117:1042. PubMed
  3. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
RRID
AB_492909 (BioLegend Cat. No. 111512)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Db on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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