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Alexa Fluor® 647 anti-mouse I-Ek/rat RT1D Antibody

Pricing & Availability
Clone
14-4-4S (See other available formats)
Regulatory Status
RUO
Other Names
I-Ek, MHC class II
Isotype
Mouse (C3H.SW) IgG2a, κ
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Product Citations
publications
14-4-4s_Alx647_020608
C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
  • 14-4-4s_Alx647_020608
    C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
110211 25 µg 101 CHF
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Description

The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1980. J. Immunol. 124:533.
  2. Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
  3. Harton JA, et al. 1991. Immunogenetics 34:358.
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596.
  5. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
  6. Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Product Citations
  1. Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
RRID
AB_493214 (BioLegend Cat. No. 110211)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14969 View all products for this Gene ID
UniProt
View information about I-Ek on UniProt.org
Go To Top Version: 3    Revision Date: 04.21.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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