Alexa Fluor 647 anti-rat CD43 Antibody anti-CD43

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Clone: W3/13 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
Isotype: Mouse IgG1, κ
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W3slash13_Alx647_021308 — LOU rat splenocytes stained with W3/13 Alexa Fluor® 647

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202810

100 µg

265 CHF

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Description

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections¹ and paraffin-embedded sections⁴.
RRID: AB_2194199 (BioLegend Cat. No. 202810)

Antigen Details

Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor: CD54, Siglec-1
Cell Type: T cells, NK cells, Granulocytes, Monocytes, Thymocytes, Platelets, Plasma cells, B cells, Hematopoietic stem and progenitors
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238

Killeen A, et al. 1987. EMBO J. 6(13):4029.

Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.

Onami TM, et al. 2002. J. Immunol. 168:6022.

Tong J, et al. 2004. J. Exp. Med. 199:1277.

Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 20737 View all products for this Gene ID
UniProt: View information about CD43 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD43 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-rat CD43	W3/13	FC
Alexa Fluor® 647 anti-rat CD43	W3/13	FC
PE anti-rat CD43	W3/13	FC
PE/Cyanine7 anti-rat CD43	W3/13	FC
PerCP/Cyanine5.5 anti-rat CD43	W3/13	FC
APC anti-rat CD43	W3/13	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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