Alexa Fluor 647 anti-rat CD86 Antibody anti-CD86

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Clone: 24F (See other available formats)
Regulatory Status: RUO
Other Names: B7-2, B70
Isotype: Mouse IgG1, κ
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24F_Alx647_091907 — ConA-stimulated (3 days) LOU rat splenocytes stained with 24F Alexa Fluor® 647

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200314

100 µg

229 CHF

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Description

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, blocking of costimulation of T cells^1-3, and immunohistochemical staining of acetone-fixed frozen sections³. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).
RRID: AB_2075123 (BioLegend Cat. No. 200314)

Antigen Details

Structure: Ig superfamily, 80 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, T cells, Antigen-presenting cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.
Gene ID: 56822 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD86 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-rat CD86	24F	FC
FITC anti-rat CD86	24F	FC
Biotin anti-rat CD86	24F	FC
Alexa Fluor® 647 anti-rat CD86	24F	FC
APC anti-rat CD86	24F	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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