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Alexa Fluor® 647 anti-STAT3 Phospho (Ser727) Antibody

Pricing & Availability
Clone
A16089B (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16089B_A647_STAT3_Antibody_080918
Human peripheral blood monocytes were treated with (filled histogram), or without (open histogram), Cell Activation Cocktail (Cat. No. 423301) for 15minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly stained with Human TruStain FcX™ (Cat. No. 422302), True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-STAT3 Phospho (Ser727) (clone A16089B) Alexa Fluor® 647.
  • A16089B_A647_STAT3_Antibody_080918
    Human peripheral blood monocytes were treated with (filled histogram), or without (open histogram), Cell Activation Cocktail (Cat. No. 423301) for 15minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly stained with Human TruStain FcX™ (Cat. No. 422302), True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-STAT3 Phospho (Ser727) (clone A16089B) Alexa Fluor® 647.
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698913 25 tests 159 CHF
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698914 100 tests 364 CHF
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Description

The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation was reported to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways.

STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The STAT3 Phospho (Ser727) antibody recognizes the regulatory serine phosphorylation of human STAT3 protein.

Product Citations
  1. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  2. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
RRID
AB_2750259 (BioLegend Cat. No. 698913)
AB_2750259 (BioLegend Cat. No. 698914)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous

Function
STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
Interaction
When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho Ser727 on UniProt.org
Go To Top Version: 1    Revision Date: 08.09.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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