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Alexa Fluor® 647 anti-TMEM119 (Extracellular) Antibody

Pricing & Availability
Clone
A16075D (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane protein 119
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A16075D_A647_TMEM119_Antibody_1_011024
Non-transfected RBL-1 cells (negative control, open histogram), or transfected with a human TMEM119 expression vector (positive control, filled histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-TMEM119 (clone A16075D) or Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400330).
  • A16075D_A647_TMEM119_Antibody_1_011024
    Non-transfected RBL-1 cells (negative control, open histogram), or transfected with a human TMEM119 expression vector (positive control, filled histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-TMEM119 (clone A16075D) or Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400330).
  • A16075D_A647_TMEM119_Antibody_2_011024
    IHC staining of Alexa Flour® 647 anti-TMEM119 (clone A16075D) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris EDTA Buffer pH 9.0, the tissue was incubated without (panel A) and with (panel B and C) 5 µg/mL of Alexa Flour® 647 anti-TMEM119 (clone A16075D). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panel A and B) and 40X (panel C) objective. Scale bar: 50 µm
  • A16075D_A647_TMEM119_Antibody_3_011024
    Non- transfected RBL-1 cells (negative control, panel A) and RBL-1 cells transfected with a human TMEM119 expression vector (positive control, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cell were stained with 1.25 µg/mL of Alexa Flour® 647 anti-TMEM119 (clone A16075D), and nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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853311 25 tests 150 CHF
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853312 100 tests 375 CHF
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853314 100 µg 550 CHF
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Description

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TMEM119 protein (extracellular domain)
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg size: 0.5 mg/mL
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC, IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and formalin-fixed paraffin-embedded immunohistochemical staining. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.

*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.

Additional Product Notes

For this antibody, we conveniently offer both microgram amounts and test sizes. Test sizes are available for convenience when using this antibody for flow cytometry/intracellular flow cytometry.

Application References

(PubMed link indicates BioLegend citation)

  1. Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed

RRID
AB_3097501 (BioLegend Cat. No. 853311)
AB_3097501 (BioLegend Cat. No. 853312)
AB_3097501 (BioLegend Cat. No. 853314)

Antigen Details

Structure
TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution

Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum.

Function
TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction
TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
  2. Satoh J, et al. 2016. Neuropathology. 36:39-49
  3. Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
  4. Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID
338773 View all products for this Gene ID
UniProt
View information about TMEM119 on UniProt.org
Go To Top Version: 1    Revision Date: 01.10.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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