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Alexa Fluor® 647 Mouse IgG3, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG3-35 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG3, κ
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Product Citations
publications
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
401321 25 µg 112 CHF
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401322 100 µg 229 CHF
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Description

The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401310) as negative control.

Product Citations
  1. Mitchell MW, et al. 2022. Stem Cell Res. 63:102833. PubMed
  2. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  3. D öpper H, et al. 2020. Stem Cell Research. 72.55486111. PubMed
  4. Neureiter A et al. 2018. Stem Cell Res. 33:20-24 . PubMed
  5. Schipper L et al. 2018. Stem Cell Res. 33:41-45 . PubMed
  6. Bi X, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.03.018. PubMed
  7. Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed
  8. Yamamoto T, et al. 2020. Stem Cell Research. 39:101485.. PubMed
  9. Menges J, et al. 2020. Stem Cell Research. 39:101517.. PubMed
RRID
AB_10683445 (BioLegend Cat. No. 401321)
AB_10683446 (BioLegend Cat. No. 401322)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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