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Alexa Fluor® 700 anti-human TCR Vα7.2 Antibody

Pricing & Availability
Clone
3C10 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor Vα7.2
Isotype
Mouse IgG1, κ
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Product Citations
publications
3C10_AF700_TCRVa7.2_Antibody_FC_1_092315
Human peripheral blood lymphocytes were stained with CD3 and TCR Vα7.2 (clone 3C10) Alexa Fluor® 700 (top), or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • 3C10_AF700_TCRVa7.2_Antibody_FC_1_092315
    Human peripheral blood lymphocytes were stained with CD3 and TCR Vα7.2 (clone 3C10) Alexa Fluor® 700 (top), or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • 3C10_AF700_TCRVa7.2_Antibody_FC_2_102315
Compare all formats See Alexa Fluor® 700 spectral data
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351727 25 tests 159 CHF
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351728 100 tests 370 CHF
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Description

The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant TCR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2  segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Martin E, et al. 2009. PLoS Biol. 7:525.
  2. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  2. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  3. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_2566336 (BioLegend Cat. No. 351727)
AB_2566336 (BioLegend Cat. No. 351728)

Antigen Details

Structure
Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution

Mucosal-associated invariant T (MAIT) cells and conventional T cells

Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.

Gene ID
6955 View all products for this Gene ID
UniProt
View information about TCR Valpha7.2 on UniProt.org
Go To Top Version: 1    Revision Date: 10.23.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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