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Anti-Alpha-I Spectrin Antibody (Previously Covance catalog# SIG-39700)

Pricing & Availability
Clone
17C7 (See other available formats)
Regulatory Status
RUO
Other Names
EL2, SPTA1, Spectrin alpha chain, erythrocyte, alpha-I spectrin, erythroid alpha-spectrin
Previously
Covance Catalog# SIG-39700
Signet Catalog# 9700-02
Signet Catalog# 9700-05
Signet Catalog# 9700-10
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
17C7_PURE_AlphaISpectrin_WB_060718
Western blot of anti-Alpha-I Spectrin antibody (clone 17C7). Lane 1: Molecular weight marker; Lane 2: 50 µg of human lung lysate; Lane 3: 50 µg of mouse lung lysate; Lane 4: 50 µg of rat brain lung lysate; Lane 5: 50 µg of human kidney lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 17C7_PURE_AlphaISpectrin_WB_060718
    Western blot of anti-Alpha-I Spectrin antibody (clone 17C7). Lane 1: Molecular weight marker; Lane 2: 50 µg of human lung lysate; Lane 3: 50 µg of mouse lung lysate; Lane 4: 50 µg of rat brain lung lysate; Lane 5: 50 µg of human kidney lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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803101 200 µL 581 CHF
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Description

Spectrin is an intracellular cytoskeletal protein that provides structure to eukaryotic cells and helps maintain plasma membrane integrity.  Alpha-1 Spectrin, encoded by the SPTA1 gene, is found primarily in erythrocytes and in the cerebellum.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant α spectrin SH3 domain
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Western Blotting (WB). Additional reported applications include immunocytochemistry1, 2.

Clone 17C7 is a specific marker for human, rat and mouse alpha I spectrin. It reacts with erythroid spectrin in red blood cells and in cerebellum. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 280 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes1, 2.

This antibody was developed against a recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Xu J, et al. 2001. Brain Res. 898(1):171-7. (WB, ICC)
  2. Xu J, et al. 2000. J. Cell Sci.113:3805-14. (WB, ICC)
Product Citations
  1. Lubbers ER, et al. 2019. J Biol Chem. 294:9576. PubMed
RRID
AB_2564659 (BioLegend Cat. No. 803101)

Antigen Details

Structure
Expected MW: 250 kD
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. De Matteis MA, Morrow JS. 2000. J. Cell Sci. 113:2331-43.
  2. Bennett V, Gilligan DM. 1993. Annu. Rev. Cell Biol. 9:27-66.
Gene ID
6708 View all products for this Gene ID 20739 View all products for this Gene ID 289257 View all products for this Gene ID
UniProt
View information about alpha-I Spectrin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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